WebMonoclonal Antibody for studying NFkB-p65 (Ser536) phosphate. Cited in 41 publications. Validated for Western Blotting, Immunoprecipitation. Highly specific and rigorously … WebAntibody was affinity purified using an epitope specific to Phospho RelA (S536) immobilized on solid support. The epitope recognized by A302-062A maps to a region of human v-rel …
Phosphorylation of RelA/p65 on Serine 536 Defines an IκBα …
WebMay 25, 2016 · Stepwise phosphorylation of p65 promotes NF-κB activation and NK cell responses during target cell recognition Nature Communications Open Access Published: 25 May 2016 Stepwise... WebThe Phospho-NFkB (Ser536) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state. chiropodists in east sussex
Cell Signaling Technology - Phospho-NF-κB p65 (Ser536) (E1Z1T) Mous…
WebJan 31, 2024 · Whole cell lysates were immunoblotted for phospho-S536 p65, phospho-S276 p65, total p65, phospho-S176/180 IKKα/β, total IKKα/β, phospho-IκBα, total IκBα, and β-actin. (B, C) IL-2-primed NK cells were mixed with autologous target cells and stimulated with plate-immobilized aKIR and/or aPD-L1 for 1 h. Cytoplasmic and nuclear extracts ... WebNo lysate preparation required. Results with as few as 10,000 cells per well. Measure two proteins simultaneously in the same well. Ideal for measuring phosphorylation. Design allows for normalization of well-to-well variations. Complete kits require minimal optimization. Suitable for high-throughput analysis of non-adherent cells. WebAntigen retrieval of a rabbit polyclonal anti-NF-κB p65 (phospho S536) was performed by incubating the sections with target retrieval solution (pH 9.0) for 20 minutes, other antibodies were performed by incubating the sections with citrate buffer (pH 6.0) for 10 minutes using pressure cooker, then the immune complex was detected with an … chiropodists in east belfast