Rk2 replicon

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August undefined, 2024

WebNov 1, 1992 · A DNA sequence analysis of seven unique mutants that are temperature sensitive for replication in Escherichia coli demonstrated that the mutants were either not … WebJul 1, 2009 · RK2 replicons potentially eliminate these types of limitations, and large-sized poorly characterized cosmid-RK2-derivatives have been used for metagenomic library …

A New and Improved Host-Independent Plasmid System for RK2 …

WebDec 1, 1992 · The RK2 replicon is not targeted by the pFREE crArray, thus omitting the CRISPR-Cas9-based self-curing feature of the pFREE system but carries a trfA mutant that … how to download brother utilities scanner

New broad-host-range promoter probe vectors based on the …

WebSpecifically, based on the broad host range RK2 replicon, we have developed a new, relatively small and well-known vector. Such vectors are useful for the construction of … Web暨南大学,数字图书馆. 开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 WebAnalysis by fluorescence microscopy has established that plasmid RK2 in Escherichia coli and other gram-negative bacteria is present as discrete clusters that are located inside … how to download brother iprint and scan

Characterizing and utilizing oxygen-dependent promoters for …

Rk2 replicon

A New and Improved Host-Independent Plasmid System for RK2 …

WebApr 11, 2024 · In order to slowly approach (and not to exceed) the correct ATPase level, its expression was driven from a low-copy broad-host range (RK2 replicon) vector (Blatny et al., 1997a, 1997b) in the E. coli K-12 MG1655 wild-type strain. WebThe minimal replicon of the broad-host-range plasmid RK2 consists of a gene, trfA (trans-acting replication), encoding a protein required for initiation of plasmid replication. The …

Rk2 replicon

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WebSep 1, 2008 · It has been demonstrated that plasmids are not randomly distributed but are located symmetrically in mid-cell, or ¼, ¾ positions in bacterial cells. In this work we … WebThe RK2 replicon plasmids produced a level of ir that was equivalent to the wild-type level (pMM4), or 2.25-fold (pPR1A6R1), 4.5-fold (pPR1A6R3), or 9.0-fold (pPR1A6) above …
WebMar 1, 2001 · The plasmid pKT240 carries the RSF1010 replicon [40] whereas the plasmid pPR9TT carries the RK2 replicon [74]. RK2 and RSF1010 are both broad host-range … WebCloned oriV can interfere with maintenance of an independent RK2 replicon. Analysis of the pTC-F14 oriV region revealed five direct repeats consisting of three perfectly conserved …
Web100% Accurate Timesheet. To ensure data is error-free and is ready for processing, Replicon timesheet software helps to verify, cleanse, analyze, review and apply the right workflows … WebThe present invention provides an expression vector comprising an RK2 minimum replicon together with an expression cassette comprising the regulatory functions of a TOL …
WebThe minimal replicon of the broad-host-range plasmid RK2 consists of a gene, trfA (trans-acting replication), encoding a protein required for initiation of plasmid replication. The …

WebOur studies have defined a plasmid segment that contains a region which must be linked in cis to the ori RK2 replicon to allow mini RK2 plasmid maintenance in P. aeruginosa PAO … least ostentatious smart watchWebNov 1, 1990 · Replicon is a(n) research topic ... Mobilization is effected by a helper plasmid consisting of the RK2 transfer genes linked to a ColE1 replicon. By use of this plasmid … least or smallestWebThe 393-base pair minimal origin,oriV, of plasmid RK2 contains three iterated motifs essential for initiation of replication: consensus sequences for binding the bacterial DnaA … how to download brothers printer driverWebFeb 6, 2014 · The replicon is comprised of the origin of replication ( ori) and all of its control elements. The ori is the place where DNA replication begins, enabling a plasmid to … least outgoing crosswordWebthe narrow host range replicon oriR6K, the sacB gene and a chloramphenicol resistance gene, using the pir-expressing E. coli strain S17-1lpir as host. The deletions within RK2 … leas totalWebJul 20, 2015 · Here, using the plasmid RK2 replicon, we analyze the protein interactions required for Escherichia coli polymerase III (Pol III) holoenzyme association at the … how to download browzwearWebApr 5, 2016 · To facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of … least outstanding requests}